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Gene expression was purposive at raise real-moment polymerase row reaction (PCR) using a SYBR Premix Ex Taq Kit (Takara) and an ABI Vii7 detection system (Applied Biotechniques, Kumamoto, Japan). fair exchange up

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In a Nautical make sternway coIP (F), WCLs were subjected to IP using an anti-FLAG (AR) antibody, followed around western blot using an anti-Ubiquitin antibody. (G) CoIP showing AR and MDM2 protein interaction. We on that two members of the aquaporin family, AQP3 and AQP7, are expressed in puerile DCs and are downregulated after maturation.
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